LOCUS       CVGEM11ZM    3223 bp    DNA             SYN       12-FEB-1996
DEFINITION  Cloning vector pGEM-11Zf(-).
ACCESSION   X65314
NID         g58166
KEYWORDS    beta-lactamase; bla gene; cloning vector; lacZ gene; multiple
            cloning site; phage f1 region; promoter; pUC/M13 primer binding
            site.
SOURCE      unidentified cloning vector.
  ORGANISM  unidentified cloning vector
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Technical,Services.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-1992) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
  REMARK    revised by [2]
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Klock,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1993) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
COMMENT     This vector can be obtained from Promega Corporation, Madison, WI.
            Call one of the following numbers for order or technical
            information:
            Order or Technical 800-356-9526
            In Wisconsin       800-356-9526
            Outside U.S.       608-274-4330
            See <X65300-X65335> for related vector sequences.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Cloning vector"
                     /plasmid="Plasmid pGEM"
     misc_feature    1
                     /note="T7 transcription initiation site"
     misc_feature    9..85
                     /note="multiple cloning sites"
     promoter        88..110
                     /note="SP6 promoter"
     misc_feature    93
                     /note="SP6 transcription initiation site"
     misc_feature    118..347
                     /note="lac operon sequence"
     misc_feature    128..144
                     /note="pUC/M13 reverse primer binding site"
     misc_feature    complement(130..132)
                     /gene="lacZ"
                     /note="lacZ start codon"
     gene            complement(130..132)
                     /gene="lacZ"
     misc_feature    152..168
                     /note="lac operator"
     gene            complement(1289..3043)
                     /gene="bla"
     misc_feature    complement(1289..3043)
                     /gene="bla"
                     /note="interuppted reading frame"
                     /product="Beta-lactamase"
     misc_feature    complement(2149..2588)
                     /gene="bla"
                     /note="phage f1 region"
     misc_feature    3044..3204
                     /note="lac operon sequence"
     misc_feature    3164..3180
                     /note="pUC/M13 forward primer binding site"
     promoter        3207..3223
                     /note="T7 promoter"
BASE COUNT      783 a    811 c    809 g    820 t
ORIGIN      
        1 gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgagggat ccgggccctc
       61 tagatgcggc cgcatgcata agcttgagta ttctatagtg tcacctaaat agcttggcgt
      121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      181 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttcgataggc
      541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1141 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1201 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1261 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1321 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1381 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1441 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1501 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1561 gtagttcgcc agttaatagt ttgcgcaacg ttgttggcat tgctacaggc atcgtggtgt
     1621 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1681 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1741 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1801 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1861 gagaataccg cgcccggcga ccgagttgct cttgcccggc gtcaatacgg gataatagtg
     1921 tatgacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1981 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2041 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2101 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2161 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2221 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2281 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2341 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2401 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2461 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2521 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2581 tcaggcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2761 cgatttagag ctttacggca cctcgaccgc aaaaaacttg atttgggtga tggttcacgt
     2821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3001 atatttaacg cgaattttaa caaaatatta acgtttacaa tttccattcg ccattcaggc
     3061 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     3121 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3181 gttgtaaaac gacggccagt gaattgtaat acgactcact ata
