LOCUS       CVGEM3ZFM    3199 bp    DNA             SYN       12-FEB-1996
DEFINITION  Cloning vector pGEM-3Zf(-).
ACCESSION   X65307
NID         g58172
KEYWORDS    beta-lactamase; bla gene; cloning vector; lacZ gene; multiple
            cloning site; phage f1 region; promoter.
SOURCE      unidentified cloning vector.
  ORGANISM  unidentified cloning vector
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Technical,Services.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-1992) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
  REMARK    revised by [3]
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Solomon,L.R., Massom,L.R. and Jarrett,H.W.
  TITLE     Enzymatic syntheses of DNA-silicas using DNA polymerase
  JOURNAL   Anal. Biochem. 203 (1), 58-69 (1992)
  MEDLINE   92398067
REFERENCE   3  (bases 1 to 3199)
  AUTHORS   Klock,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1993) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
COMMENT     This vector can be obtained from Promega Corporation, Madison, WI.
            Call one of the following numbers for order or technical
            information:
            Order or Technical 800-356-9526
            In Wisconsin       800-356-9526
            Outside U.S.       608-274-4330
            See <X65300-X65335> for related vector sequences.
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Cloning vector"
                     /plasmid="Plasmid pGEM"
     misc_feature    1
                     /note="T7 transcription initiation site"
     misc_feature    5..61
                     /note="multiple cloning sites"
     promoter        64..86
                     /note="SP6 promoter"
     misc_feature    69
                     /note="SP6 transcription initiation site"
     misc_feature    94..323
                     /note="lac operon sequence"
     misc_feature    complement(106..108)
                     /gene="lacZ"
                     /note="lacZ start codon"
     gene            complement(106..108)
                     /gene="lacZ"
     gene            complement(1265..2125)
                     /gene="bla"
     CDS             complement(1265..2125)
                     /gene="bla"
                     /codon_start=1
                     /product="Beta-lactamase"
                     /db_xref="PID:g58173"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    2564..3019
                     /note="phage f1 region"
     misc_feature    3020..3180
                     /note="lac operon sequence"
     promoter        3183..3199
                     /note="T7 promoter"
BASE COUNT      779 a    803 c    801 g    816 t
ORIGIN      
        1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
       61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      481 gtaaaaaggc cgcgttgctg gcgtttttcg ataggctccg cccccctgac gagcatcaca
      541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1561 gcaacgttgt tggcattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga ataccgcgcc cggcgaccga
     1861 gttgctcttg cccggcgtca atacgggata atagtgtatg acatagcaga actttaaaag
     1921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2281 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2461 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2521 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgacgcgcc ctgtagcggc
     2581 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2641 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2701 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc
     2761 gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2821 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2881 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2941 tcggcctatt ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa
     3001 atattaacgt ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3061 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3121 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3181 tgtaatacga ctcactata
