LOCUS       CVGEM5ZFM    3003 bp    DNA             SYN       12-FEB-1996
DEFINITION  Cloning vector pGEM-5Zf(-).
ACCESSION   X65309
NID         g58174
KEYWORDS    beta-lactamase; bla gene; cloning vector; lacZ gene; multiple
            cloning site; phage f1 region; promoter; pUC/M13 primer binding
            site.
SOURCE      unidentified cloning vector.
  ORGANISM  unidentified cloning vector
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Technical,Services.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-1992) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
  REMARK    revised by [2]
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Klock,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1993) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
COMMENT     This vector can be obtained from Promega Corporation, Madison, WI.
            Call one of the following numbers for order or technical
            information:
            Order or Technical 800-356-9526
            In Wisconsin       800-356-9526
            Outside U.S.       608-274-4330
            See <X65300-X65335> for related vector sequences.
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Cloning vector"
                     /plasmid="Plasmid pGEM"
     misc_feature    1
                     /note="T7 transcription initiation site"
     misc_feature    10..113
                     /note="multiple cloning sites"
     promoter        121..143
                     /note="SP6 promoter"
     misc_feature    126
                     /note="SP6 transcription initiation site"
     misc_feature    151..201
                     /note="lac operator"
     misc_feature    151..180
                     /note="lac operon sequence"
     misc_feature    161..177
                     /note="pUC/M13 reverse primer binding site"
     gene            complement(163..165)
                     /gene="lacZ"
     misc_feature    complement(163..165)
                     /gene="lacZ"
                     /note="lacZ start codon"
     gene            complement(1322..2182)
                     /gene="bla"
     CDS             complement(1322..2182)
                     /gene="bla"
                     /codon_start=1
                     /product="Beta-lactamase"
                     /db_xref="PID:g58175"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    2368..2823
                     /note="phage f1 region"
     misc_feature    2824..2984
                     /note="lac operon sequence"
     misc_feature    2944..2960
                     /note="pUC/M13 forward primer binding site"
     promoter        2987..3003
                     /note="T7 promoter"
BASE COUNT      726 a    761 c    746 g    770 t
ORIGIN      
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
       61 gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
      121 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      541 aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa
      601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      961 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1621 acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1861 ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt
     1921 gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc
     1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2281 ttccgcgcac atttccccga aaagtgccac ctgtatgcgg tgtgaaatac cgcacagatg
     2341 cgtaaggaga aaataccgca tcaggcgacg cgccctgtag cggcgcatta agcgcggcgg
     2401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2521 gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc aaaaaacttg
     2581 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2701 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2761 aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta acgtttacaa
     2821 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2881 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2941 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     3001 ata
