LOCUS       CVGEM9ZFM    2925 bp    DNA             SYN       12-FEB-1996
DEFINITION  Cloning vector pGEM-9Zf(-).
ACCESSION   X65312
NID         g58182
KEYWORDS    beta-lactamase; bla gene; cloning vector; lacZ gene; multiple
            cloning site; phage f1 region; promoter; pUC/M13 primer binding
            site.
SOURCE      unidentified cloning vector.
  ORGANISM  unidentified cloning vector
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Technical,Services.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-1992) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
  REMARK    revised by [2]
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Klock,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1993) Technical Services, Promega Corporation,
            2800 Woods Hollow Road, Madison, Wi 53711-5399, USA
COMMENT     This vector can be obtained from Promega Corporation, Madison, WI.
            Call one of the following numbers for order or technical
            information:
            Order or Technical 800-356-9526
            In Wisconsin       800-356-9526
            Outside U.S.       608-274-4330
            See <X65300-X65335> for related vector sequences.
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Cloning vector"
                     /plasmid="Plasmid pGEM"
     misc_feature    1
                     /note="SP6 transcription initiation site"
     misc_feature    5..54
                     /note="multiple cloning sites"
     promoter        50..72
                     /note="T7 promoter"
     misc_feature    55
                     /note="T7 transcription initiation site"
     misc_feature    93..322
                     /note="lac operon sequence"
     misc_feature    103..119
                     /note="pUC/M13 reverse primer binding site"
     gene            complement(105..107)
                     /gene="lacZ"
     misc_feature    complement(105..107)
                     /gene="lacZ"
                     /note="lacZ start codon"
     misc_feature    127..143
                     /note="lac operator"
     gene            complement(1264..2124)
                     /gene="bla"
     CDS             complement(1264..2124)
                     /gene="bla"
                     /codon_start=1
                     /product="Beta-lactamase"
                     /db_xref="PID:g58183"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    2256..2722
                     /note="phage f1 region"
     misc_feature    2724..2881
                     /note="lac operon sequence"
     misc_feature    2844..2860
                     /note="pUC/M13 reverse primer binding site"
     promoter        2909..2925
                     /note="SP6 promoter"
BASE COUNT      714 a    740 c    711 g    760 t
ORIGIN      
        1 gaatatgcat cactagtaag ctttgctcta gactggaatt cgtcgacgag ctccctatag
       61 tgagtcgtat tagaggccga cttggccaaa ttcgtaatca tggtcatagc tgtttcctgt
      121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
      961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     2281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2401 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2461 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2521 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2581 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2641 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2701 aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     2761 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     2821 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     2881 gccaagctga cttggtcagc ggccgcagat ttaggtgaca ctata
