LOCUS       SYNAA113M    9186 bp    DNA             SYN       29-SEP-1995
DEFINITION  Sequencing vector pAA113M DNA.
ACCESSION   M20189
NID         g207851
KEYWORDS    cloning vector; synthetic DNA.
SOURCE      Synthetic DNA.
  ORGANISM  artificial sequence
            artificial sequence.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ahmed,A.
  TITLE     A vector for sequencing long (40-kb) DNA fragments
  JOURNAL   Gene 75 (2), 315-321 (1989)
  MEDLINE   89232762
COMMENT     Draft entry and computer readable copy of sequence [1] kindly
            provided by A.Ahmed, 28-SEP-1988.  pAA113M  is a cosmid vector
            specially developed to sequence long (40kb) DNA fragments.  It
            makes use of the cos site to clone long segments, IS1 to subdivide
            the cloned fragment by deletions, galKT to select such deletions,
            and some unique restriction sites to further shorten the fragment
            for supercoil or M13 sequencing.  It contains a normal plasmid ori
            of replication and also the M13 ori of replication to obtain DNA in
            the double-stranded or single- stranded form.
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="artificial sequence"
     CDS             3787..4977
                     /codon_start=1
                     /product="tet protein"
                     /db_xref="PID:g207852"
                     /transl_table=11
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
     CDS             complement(7433..8293)
                     /note="putative"
                     /codon_start=1
                     /product="amp protein"
                     /db_xref="PID:g1004350"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT     2051 a   2446 c   2453 g   2236 t
ORIGIN      Unreported.
        1 caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa
       61 gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg
      121 ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct
      181 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
      241 ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg
      301 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
      361 cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat
      421 aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc
      481 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
      541 taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
      601 gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
      661 gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
      721 tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
      781 gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
      841 ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg
      901 ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt
      961 ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt
     1021 tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg
     1081 ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga
     1141 gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc
     1201 atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg
     1261 cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc
     1321 gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc
     1381 gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg
     1441 aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc
     1501 agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga
     1561 tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg
     1621 atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact
     1681 tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg
     1741 tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag
     1801 ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc
     1861 tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg
     1921 atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt
     1981 tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat
     2041 gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg
     2101 cgaaaatgga tatcgctgac tgcgcgcaaa cgctctgctg cctgttctgc ggtcaggtct
     2161 cgctgggtct ctgccagcat ttcataacca accataaatt tacgtacggt ggcggacggc
     2221 agcagaggcg gataaaagtg cgcgtgcagc tgccagtgtt gattctcttc gccattaaat
     2281 ggcgcgccgt gccagcccat agagtagggg aaggagcact ggaagaggtt gtcataacga
     2341 ctggtcagct ttttcaacgc cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg
     2401 atccgtaaaa cgtgggcttt gggcagcagt agcgtttcga acggccaggc agcccagtaa
     2461 ggcacgacgg ctaaccagtg ttcggtttcg acaacggtac ggctaccgtc tgccagctcg
     2521 cgctgaacat aatccaccag cattggtgat ttctgttcgg caaaatattc tttttgcagg
     2581 cggtcttcgc gctcagcttc gttaggcagg aagctatttg cccaaatctg accgtgcgga
     2641 tgcgggttag agcagcccat cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac
     2701 gttttcccca gttctgcggt ttgctcctgc caggttttga cgatttccgt caatgctgca
     2761 acgctgagct ctggcagcgt tttactgtga tccggtgaaa agcagatcac ccggctggtg
     2821 ccgcgcgcgc tctggcaacg catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca
     2881 gacatcaaag ccgcaaagtc attagtgaaa acgtaagtcc cggtgtaatc ggggttttta
     2941 tcgcctgtca cccgcacatt acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac
     3001 acctgtttgg ctggcgtttc ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac
     3061 cagaatccat tgcccggtga gcggttgtag cggcgatgtg gatgatcaac gggattaaat
     3121 tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg ccagtgccag
     3181 gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg
     3241 ctggcgtccg ccagtaggcc ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac
     3301 cggtttgccg cacgtttgct gaaggcatta accacgtcca gcacgctgtt gcctacgcca
     3361 gcgccgaggt tgtagatgtg tacgcctggc ttgttcgcca gtttttccat cgccacgacg
     3421 tgaccgtccg ccagatccat tacgtggatg taatcgcgta cgccagtacc atcttcggtc
     3481 ggataatcgt taccaaaaat cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg
     3541 tatggcatca ggttattcgg aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc
     3601 gcgccaaccg ggttgaagta gcgcacgagg gcaatgctcc agtccggctg ggctttttgc
     3661 agatcggtga ggatctgttc caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg
     3721 ccggtcggga agctataatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     3781 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     3841 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
     3901 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     3961 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     4021 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc
     4081 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     4141 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     4201 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     4261 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     4321 ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
     4381 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     4441 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     4501 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     4561 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     4621 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     4681 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     4741 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     4801 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     4861 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     4921 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     4981 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     5041 tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     5101 tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
     5161 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     5221 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     5281 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     5341 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     5401 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     5461 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     5521 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     5581 ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     5641 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     5701 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     5761 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5821 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5881 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     5941 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     6001 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     6061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6901 agattatcaa aaaggatctt cacctagatc cttttaaatt gtaaacgtta atattttgtt
     6961 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7021 caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
     7081 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7141 tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
     7201 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7261 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7321 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7381 acagggcgcg taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7741 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8041 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8401 catttccccg aaaagtgcgg taatgactcc aacttattga tagtgtttta tgttcagata
     8461 atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc
     8521 cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg
     8581 cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt
     8641 catccatatc accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat
     8701 agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa
     8761 cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca
     8821 gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt
     8881 aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca
     8941 acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa
     9001 gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg
     9061 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
     9121 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
     9181 atcacc
